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superscript ii rnaseh - reverse transcriptase  (Thermo Fisher)


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    Thermo Fisher superscript ii rnaseh - reverse transcriptase
    Superscript Ii Rnaseh Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superscript+ii+rnaseh+reverse+transcriptase/pmc00126259-235-37-43
    Average 90 stars, based on 1 article reviews
    superscript ii rnaseh - reverse transcriptase - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Synthesized:

    Article Title: Elevated Stearoyl-CoA Desaturase in Brains of Patients with Alzheimer's Disease
    Article Snippet: RNA quality was assessed using an Agilent BioAnalyzer and by UV spectrophotometry. .. First-strand complementary DNAs were synthesized using SuperScript II RNaseH reverse transcriptase (Invitrogen). .. Reverse transcription of total RNA (2 μg) was carried out using oligo(dT)12–18 primers for 50 min at 42°C. mRNA levels were measured by quantitative real-time polymerase chain reaction (RT-PCR) with a Mx 3000P system (Stratagene, La Jolla, CA).

    Article Title: Genomic Imprinting of IGF2 Is Maintained in Infantile Hemangioma despite its High Level of Expression
    Article Snippet: Cryosections of tumor and normal tissues were either fixed in 4% paraformaldehye and labeled with mouse anti-human IGF2 (UBI, Lake Placid, NY, USA), or fixed in acetone and stained with rabbit anti-human GLUT1 (Dako, Carpinteria, CA, USA). .. cDNA was synthesized from total RNA isolated from human full-term placenta, neonatal foreskin, proliferating hemangioma, lymphatic malformation, venous malformation, pyogenic granuloma, vascular tumors known as hemangioendothelioma, and human dermal microvascular endothelial cells with Superscript II RnaseH Reverse Transcriptase (Invitrogen, Carlsbad, CA, USA). ..

    Article Title: Genomic Imprinting of IGF2 Is Maintained in Infantile Hemangioma despite its High Level of Expression
    Article Snippet: Cryosections of tumor and normal tissues were either fixed in 4% paraformaldehye and labeled with mouse anti-human IGF2 (UBI, Lake Placid, NY, USA), or fixed in acetone and stained with rabbit anti-human GLUT1 (Dako, Carpinteria, CA, USA). .. Quantitative RT-PCR cDNA was synthesized from total RNA isolated from human full-term placenta, neonatal foreskin, proliferating hemangioma, lymphatic malformation, venous malformation, pyogenic granuloma, vascular tumors known as hemangioendothelioma, and human dermal microvascular endothelial cells with Superscript II RnaseH Reverse Transcriptase (Invitrogen, Carlsbad, CA, USA). ..

    Reverse Transcription:

    Article Title: Elevated Stearoyl-CoA Desaturase in Brains of Patients with Alzheimer's Disease
    Article Snippet: RNA quality was assessed using an Agilent BioAnalyzer and by UV spectrophotometry. .. First-strand complementary DNAs were synthesized using SuperScript II RNaseH reverse transcriptase (Invitrogen). .. Reverse transcription of total RNA (2 μg) was carried out using oligo(dT)12–18 primers for 50 min at 42°C. mRNA levels were measured by quantitative real-time polymerase chain reaction (RT-PCR) with a Mx 3000P system (Stratagene, La Jolla, CA).

    Article Title: Genomic Imprinting of IGF2 Is Maintained in Infantile Hemangioma despite its High Level of Expression
    Article Snippet: Cryosections of tumor and normal tissues were either fixed in 4% paraformaldehye and labeled with mouse anti-human IGF2 (UBI, Lake Placid, NY, USA), or fixed in acetone and stained with rabbit anti-human GLUT1 (Dako, Carpinteria, CA, USA). .. cDNA was synthesized from total RNA isolated from human full-term placenta, neonatal foreskin, proliferating hemangioma, lymphatic malformation, venous malformation, pyogenic granuloma, vascular tumors known as hemangioendothelioma, and human dermal microvascular endothelial cells with Superscript II RnaseH Reverse Transcriptase (Invitrogen, Carlsbad, CA, USA). ..

    Article Title: Green tea powder and Lactobacillus plantarum affect gut microbiota, lipid metabolism and inflammation in high-fat fed C57BL/6J mice
    Article Snippet: Total RNA was isolated from grounded liver (see above) using the RNeasy Mini Plus Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instruction. .. Total RNA (1 μg) was treated with DNaseI amplification grade (Invitrogen, Carlsbad, CA, USA) and reversely transcribed using random hexamers (Amersham Bioscieces, Piscataway, NJ, USA) and SuperScript II RNaseH reverse transcriptase (Invitrogen) according to the manufacturer’s recommendations. .. The cDNA was used for quantitative PCR using TaqMan chemistry (assays-on-demand, Applied Biosystems) or SYBR green chemistry (primers were designed using the software Primer Express, Applied Biosystems), with an ABI 7900 system (Applied Biosystem, Foster City, CA, USA), see Additional file for assay IDs of the Taqman probes and list of primer sequences.

    Article Title: Nutritional Programming in the Rat Is Linked to Long-Lasting Changes in Nutrient Sensing and Energy Homeostasis in the Hypothalamus
    Article Snippet: .. 2 μg of purified RNA extracted from the whole hypothalamus of 35 and 180 days-old animals, was reverse transcribed using Superscript II RNAseH- Reverse-Transcriptase (Invitrogen) in a total volume of 20 μl. ..

    Article Title: MITEs in the promoters of effector genes allow prediction of novel virulence genes in Fusarium oxysporum
    Article Snippet: From the collected material, RNA was isolated using TRIzol reagent (Gibco) followed by phenol-chloroform extraction. .. The isolated RNA was used to make cDNA using Promega Rnasin (ribonuclease inhibitor) and Gibco Superscript II RNaseH Reverse transcriptase according to the manufacturer’s instructions. ..

    Article Title: Genomic Imprinting of IGF2 Is Maintained in Infantile Hemangioma despite its High Level of Expression
    Article Snippet: Cryosections of tumor and normal tissues were either fixed in 4% paraformaldehye and labeled with mouse anti-human IGF2 (UBI, Lake Placid, NY, USA), or fixed in acetone and stained with rabbit anti-human GLUT1 (Dako, Carpinteria, CA, USA). .. Quantitative RT-PCR cDNA was synthesized from total RNA isolated from human full-term placenta, neonatal foreskin, proliferating hemangioma, lymphatic malformation, venous malformation, pyogenic granuloma, vascular tumors known as hemangioendothelioma, and human dermal microvascular endothelial cells with Superscript II RnaseH Reverse Transcriptase (Invitrogen, Carlsbad, CA, USA). ..

    Article Title: Molecular Evolution of Human Immunodeficiency Virus Type 1 upon Transmission between Human Leukocyte Antigen Disparate Donor-Recipient Pairs
    Article Snippet: .. Viral RNA was isolated from plasma or serum using the QIAgen Viral RNA Mini Kit and reverse transcribed into cDNA with Superscript II RnaseH Reverse Transcriptase (Invitrogen) using outer primer seq2 ( 5′-TCCCTCATATCTCCTCCTCCAGGTC-3′ ). ..

    Isolation:

    Article Title: Genomic Imprinting of IGF2 Is Maintained in Infantile Hemangioma despite its High Level of Expression
    Article Snippet: Cryosections of tumor and normal tissues were either fixed in 4% paraformaldehye and labeled with mouse anti-human IGF2 (UBI, Lake Placid, NY, USA), or fixed in acetone and stained with rabbit anti-human GLUT1 (Dako, Carpinteria, CA, USA). .. cDNA was synthesized from total RNA isolated from human full-term placenta, neonatal foreskin, proliferating hemangioma, lymphatic malformation, venous malformation, pyogenic granuloma, vascular tumors known as hemangioendothelioma, and human dermal microvascular endothelial cells with Superscript II RnaseH Reverse Transcriptase (Invitrogen, Carlsbad, CA, USA). ..

    Article Title: MITEs in the promoters of effector genes allow prediction of novel virulence genes in Fusarium oxysporum
    Article Snippet: From the collected material, RNA was isolated using TRIzol reagent (Gibco) followed by phenol-chloroform extraction. .. The isolated RNA was used to make cDNA using Promega Rnasin (ribonuclease inhibitor) and Gibco Superscript II RNaseH Reverse transcriptase according to the manufacturer’s instructions. ..

    Article Title: Genomic Imprinting of IGF2 Is Maintained in Infantile Hemangioma despite its High Level of Expression
    Article Snippet: Cryosections of tumor and normal tissues were either fixed in 4% paraformaldehye and labeled with mouse anti-human IGF2 (UBI, Lake Placid, NY, USA), or fixed in acetone and stained with rabbit anti-human GLUT1 (Dako, Carpinteria, CA, USA). .. Quantitative RT-PCR cDNA was synthesized from total RNA isolated from human full-term placenta, neonatal foreskin, proliferating hemangioma, lymphatic malformation, venous malformation, pyogenic granuloma, vascular tumors known as hemangioendothelioma, and human dermal microvascular endothelial cells with Superscript II RnaseH Reverse Transcriptase (Invitrogen, Carlsbad, CA, USA). ..

    Article Title: Molecular Evolution of Human Immunodeficiency Virus Type 1 upon Transmission between Human Leukocyte Antigen Disparate Donor-Recipient Pairs
    Article Snippet: .. Viral RNA was isolated from plasma or serum using the QIAgen Viral RNA Mini Kit and reverse transcribed into cDNA with Superscript II RnaseH Reverse Transcriptase (Invitrogen) using outer primer seq2 ( 5′-TCCCTCATATCTCCTCCTCCAGGTC-3′ ). ..

    Amplification:

    Article Title: Green tea powder and Lactobacillus plantarum affect gut microbiota, lipid metabolism and inflammation in high-fat fed C57BL/6J mice
    Article Snippet: Total RNA was isolated from grounded liver (see above) using the RNeasy Mini Plus Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instruction. .. Total RNA (1 μg) was treated with DNaseI amplification grade (Invitrogen, Carlsbad, CA, USA) and reversely transcribed using random hexamers (Amersham Bioscieces, Piscataway, NJ, USA) and SuperScript II RNaseH reverse transcriptase (Invitrogen) according to the manufacturer’s recommendations. .. The cDNA was used for quantitative PCR using TaqMan chemistry (assays-on-demand, Applied Biosystems) or SYBR green chemistry (primers were designed using the software Primer Express, Applied Biosystems), with an ABI 7900 system (Applied Biosystem, Foster City, CA, USA), see Additional file for assay IDs of the Taqman probes and list of primer sequences.

    Purification:

    Article Title: Nutritional Programming in the Rat Is Linked to Long-Lasting Changes in Nutrient Sensing and Energy Homeostasis in the Hypothalamus
    Article Snippet: .. 2 μg of purified RNA extracted from the whole hypothalamus of 35 and 180 days-old animals, was reverse transcribed using Superscript II RNAseH- Reverse-Transcriptase (Invitrogen) in a total volume of 20 μl. ..

    Quantitative RT-PCR:

    Article Title: Genomic Imprinting of IGF2 Is Maintained in Infantile Hemangioma despite its High Level of Expression
    Article Snippet: Cryosections of tumor and normal tissues were either fixed in 4% paraformaldehye and labeled with mouse anti-human IGF2 (UBI, Lake Placid, NY, USA), or fixed in acetone and stained with rabbit anti-human GLUT1 (Dako, Carpinteria, CA, USA). .. Quantitative RT-PCR cDNA was synthesized from total RNA isolated from human full-term placenta, neonatal foreskin, proliferating hemangioma, lymphatic malformation, venous malformation, pyogenic granuloma, vascular tumors known as hemangioendothelioma, and human dermal microvascular endothelial cells with Superscript II RnaseH Reverse Transcriptase (Invitrogen, Carlsbad, CA, USA). ..

    Clinical Proteomics:

    Article Title: Molecular Evolution of Human Immunodeficiency Virus Type 1 upon Transmission between Human Leukocyte Antigen Disparate Donor-Recipient Pairs
    Article Snippet: .. Viral RNA was isolated from plasma or serum using the QIAgen Viral RNA Mini Kit and reverse transcribed into cDNA with Superscript II RnaseH Reverse Transcriptase (Invitrogen) using outer primer seq2 ( 5′-TCCCTCATATCTCCTCCTCCAGGTC-3′ ). ..

    other:

    Article Title: Attainment of Brown Adipocyte Features in White Adipocytes of Hormone-Sensitive Lipase Null Mice
    Article Snippet: Total RNA (1 μg) was treated with DNase I (DNase I amplification grade, Invitrogen) and then reversely transcribed using random hexamers (Amersham Biosciences) and SuperScriptII RNaseH reverse transcriptase (Invitrogen Life Technologies) according to the manufacturer's recommendations.



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